Effect of Genotype, Explant Size and Position on Callus Induction in Cereus Peruvianus Mill. (cactaceae)

نویسندگان

  • N. Karimi
  • M. R. Mofid
  • M. Ebrahimi
  • S. M. Khayyam Nekouei
  • Mohammad reza Mofid
چکیده

Cereus peruvianus (Cactaceae) is an important medical plant. The study was carried out on callus induction of Cereus jamacaru f. monstrosus and Cereus hildmannianus fma monstrosa (Cereus peruvianus). Apical and lateral explants were cultured on Murashige and Skoog media with factorial combinations of the auxins indole-3acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and N-(2-furanyl-methyl)-1-purine-6 amine (kinetin) at the concentrations 1, 2, 3, 4 mg/l . Positive results were obtained from the apical and lateral explants in all conditions tested, but apical explants gave the highest callus induction in all growth regulator combinations tested. Explants’ size doesn’t have a significant impact.

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تاریخ انتشار 2010